Journal: PLoS ONE
Article Title: Lymphatic Involvement in the Disappearance of Steroidogenic Cells from the Corpus Luteum during Luteolysis
doi: 10.1371/journal.pone.0088953
Figure Lengend Snippet: A: Large lymphatic vessels (black arrowheads) were observed around arterioles (yellow arrowheads). B: Negative control. C: Lymphatic vessels were visualized by LYVE-1 antibody and goat anti rabbit IgG antibody labeled with Alexa 488 (white arrowheads). D: Steroidogenic cells were detected by 3β-HSD antibody and goat anti mouse IgG antibody labeled with Alexa 594 (blue arrowheads). E: Many 3β-HSD positive cell were observed in the lumen of LYVE-1 positive vessel (merge C and D). LYVE-1 antibody positively reacted with 3β-HSD positive cells (red arrowheads). F: Negative control. Nuclei were stained using DAPI. All scale bars, 50 µm.
Article Snippet: Subsequently, sections were incubated with ImmPRESS UNIVERSAL reagent, anti-mouse/rabbit Ig (MP-7500; Vector Laboratories Inc, Burlingame, CA, USA) for 30 min according to the manufacturer’s instructions.
Techniques: Negative Control, Labeling, Staining